Monday, March 10, 2008
Real Time Thermocycler -- Seems ok
Our rep came today to check the machine due to our strange problem with the strip caps on the plate. He couldn't find anything wrong with the machine, especially the alignment. He did measure the block temperature and said it was a little hot (106 when it should be 105), nothing to worry about, and he adjusted it. He also found some contamination in four of the wells and cleaned that. He recommends we always use film with the plates and let him know if there are any further problems. He also noticed that many of the plates in the trash can by the machine have scratches all over the tops of the caps. If this was like this during the run, it would cause artifacts. We couldn't figure out where the scratches were coming from. Let me know if you know anything about this.
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