A user reported to me that she noticed among many z-stacks in a row that a few times the z focus gears seemed to stick, such that 3 or 4 images were clearly in the same focal plane rather than different ones, but then the focus would change. She thinks it's a sporadic, unpredictable event, and didn't save the .ids files so that we could examine the images (she renders them and saves the resulting image).
I contacted our service rep, Steve Fryer, and he'll be coming to take a look on Wednesday afternoon next week. My recommendation for users is to watch your scans in case the focus motor behaves erratically, carefully engage the focus motor and check by trying to move the fine focus knob (gently), and SAVE .ids FILES FOR YOUR Z-STACKS. As I've probably mentioned to most users, even though they're large, they keep all of your data, so that you can judge if anything has gone awry, redo your projections, make new movies, make scale bars, and pick out the settings you used, just to name a few of the important reasons to save your .ids files.
If you are concerned about the amount of disk space this would take up, remember that DVDs are less than a dollar apiece and can store over 4 GB of data, which can be burning while you clean off the objectives at the end of your session. Talk to me if you have any questions or concerns about this.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment